Complement C4d (C4d) Antibody

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Catalogue No: abx227114
Price: US$290.00
(Size: 40 µl)
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IHC-P analysis of transplanted kidney tissue (4 µm section) stained with anti-C4d complement antibody, showing diffuse strong positive immunostaining of peritubular and glomerular capillaries, indicating acute antibody mediated rejection.
Complement C4d (C4d) Antibody Complement C4d (C4d) Antibody

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Datasheet SDS
Complement C4d (C4d) Antibody is a Rabbit Monoclonal antibody for the detection of C4d complement.

Target Complement C4d (C4d)
Clonality Monoclonal
Clone E588
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the internal region of human C4.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P0C0L4 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Concentration 1 mg/ml
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Dissolve 1.21 g Tris and 0.37 g EDTA in 700 ml of distilled water and mix. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Wash Buffer: Use 0.05 M Tris-HCl, pH 7.6, containing 0.2% Tween-20; or PBS containing 0.2% Tween-20.
  3. Preparation of Copper Sulfate Solution: Dissolve 0.90 g NaCl and 0.50 g CuSO4.5H2O in 100 ml distilled water.
  4. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  5. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  6. Rinse twice in distilled water, 5 minutes each.
  7. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  8. Rinse twice in distilled water, 5 minutes each.
  9. Antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, and incubate in a water bath for 20-25 minutes at 96-98 °C.
  10. Remove the slide from the water bath and allow to stand at room temperature (in Tris-EDTA buffer, pH 9.0) for 20 minutes.
  11. Rinse twice in distilled water, 5 minutes each.
  12. Wash twice in Wash Buffer, 5 minutes each.
  13. Incubate the section with primary antibody at 1/100 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (≥ 1 mg/ml) to dilute this antibody.
  14. Rinse three times in distilled water, 5 minutes each.
  15. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  16. Wash three times in Wash Buffer, 5 minutes each.
  17. Apply the DAB chromagen for 1-3 minutes.
  18. Wash twice in distilled water, 5 minutes each.
  19. Rinse in Copper Sulfate Solution.
  20. Wash in distilled water for 2 minutes.
  21. Stain in hematoxylin for 5 minutes.
  22. Wash three times in distilled water, 2 minutes each.
  23. Rinse in 37 mM ammonium hydroxide solution.
  24. Wash in distilled water for 2 minutes.
  25. Mount the slide for observation.
Research Articles on Complement C4d (C4d)


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